​Cloning and Sequence Analysis of Transferrin Binding Protein Gene A of Field and Vaccine Strains of Pasteurella multocida B:2

Background: Pasteurella multocida is an important bacterial pathogen that causes many major diseases of which haemorrhagic septiciemia (HS) in cattle and buffalo responsible for catastrophic epizootics India South Asia. In India, the disease considered as most dreaded disease. Various host- pathogen- specific determinants are outcome. virulence genes (tbpA, pfhA, toxA, hgbB, hgbA, nanH, nanB, sodA, sodC, oma87 ptfA) have been proposed to play a key role this interaction. Methods: The present study was done compare gene deduced amino acid sequence transferrin binding protein (tbpA) field isolates vaccine strain P. B: 2. Result: It observed tbpA strains similar nucleotide except at positions 574 620. used prediction matured TbpA characteristics. sequences 242 acids revealed 99% homology with variety other TonB-dependent receptor proteins, indicating it belongs family outer membrane receptors. Deduced found be 207th acid. Leucine there while Phenyl alanine found. These both hydrophobic nature so no change physico-chemical property expected. From concluded single difference between isolate might not cause its property.